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Glycogen synthase kinase 3#beta# induces cell cycle arrest in a cyclin D1--dependent manner in human lung adenocarcinoma cell line A549

Li Jiansha, Zhu Min, Tian Dan, Wang Manxiang, Wang Fang, Li Naping, Wu Renliang

Department of Pathology,Tongji Medical College,Huazhong University of Science and Technology,and Key Laboratory of Pulmonary Disease of Ministry of Health of China.Wuhan 430030,Hubei

Abstract

The effect of glycogen synthase kinase 3#beta# (GSK3#beta#) has been repeatedly implicated in cell proliferation, but studies on the effect of GSK3#beta# in different cell lines with different stimuli have drawn different conclusions. To investigate the direct effect of GSK3#beta# on cell growth in human lung adenocarcinoma cell line A549, we changed its activity by transient transfection with two kinds of GSK3#beta# mutant plasmids, constitutively active form S9A-GSK3#beta# and dominant negative form KM-GSK3#beta#. Twenty-four hours later, cell counting, flow cytometry and Western blot detection were made respectively. The results showed that enhancing GSK3#beta# activity caused a decrease in cell number, as well as a higher percentage of cells at G_(1) phase. Further, the expression of cyclin D1 was downregulated by GSK3#beta#. Taken together, our observations suggest that GSK3#beta# may induce G_(1) cell cycle arrest in a cyclin D1-dependent fashion and therefore possibly plays a growth-inhibitory role in A549 cells.

Key words: glycogen synthase kinase 3#beta#;Cell proliferation;Cell cycle;cyclin D1

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Citing This Article：

Li Jiansha, Zhu Min, Tian Dan, Wang Manxiang, Wang Fang, Li Naping, Wu Renliang. Glycogen synthase kinase 3#beta# induces cell cycle arrest in a cyclin D1--dependent manner in human lung adenocarcinoma cell line A549. Acta Physiol Sin 2007; 59 (2): 204-209 (in Chinese with English abstract).