Issue Archive

Recombinant soluble receptor activator of nuclear factor--#kappa#B inhibits parathyroid hormone--induced osteoclastogenesis {sl in vitro}

Wang Baoli, Liang Hui, Zheng Fang, Li Xiaoxia, Liu Yubing, Dai Chenlin

Institute of Endocrinology,Tianjin Medical University.Tianjin 300070；China；Division of Endocrinology, Tianjin Medical University General Hospital.Tianjin 300052

Abstract

The recent identification of receptor activator of nuclear factor-#kappa#B ligand (RANKL)/RANK/osteoprotegerin (OPG) cytokine system has led to a new molecular perspective on osteoclast biology and bone homeostasis. Specifically, the interaction between RANKL and RANK is responsible for osteoclast differentiation. In the present study, we evaluated whether soluble RANK (sRANK)could act as an antagonist of RANKL and down-regulate osteoclastogenesis and bone resorption in vitro. The prokaryotic expression vector coding for sRANK was constructed. Then the construct was introduced into E. coli Origami B (DE3) competent cells and recombinant sRANK was successfully produced and purified through affinity chromatography. sRANK reduced osteoclast-like cell (OLC) formation and resorption pit formation induced by parathyroid hormone (PTH) in a dose-dependent manner. In addition,sRANK significantly inhibited PTH-induced mRNA expression of carbonic anhydrase Ⅱ and tartrate-resistant acid phosphatase in murine bone marrow cells as confirmed by using semi-quantitative RT-PCR. The down-regulation was highly correlated with the effect of sRANK on OLC formation from marrow cells. These data demonstrate the anti-resorptive effects of sRANK in vitro and highlight the potential of sRANK as a novel therapeutic approach to bone disorders characterized by enhanced bone resorption.

Key words: receptor activator of nuclear factor-#kappa#B;osteoclasts;in vitro

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Wang Baoli, Liang Hui, Zheng Fang, Li Xiaoxia, Liu Yubing, Dai Chenlin. Recombinant soluble receptor activator of nuclear factor--#kappa#B inhibits parathyroid hormone--induced osteoclastogenesis {sl in vitro}. Acta Physiol Sin 2007; 59 (2): 169-174 (in Chinese with English abstract).