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Calreticulin expression increases during delayed cardioprotection induced by hypoxic preconditioning

Xu Feifei, Fu Yan, Liu Fengying, Zhu Xiaomei, Liu Xiuhua

Department of Pathophysiology, Chinese People's Liberation Army General Hospital.Beijing 100853

Abstract

Both in rive and cultured cardiomyocyte experiments were performed to investigate the alteration of expression of calreticulin （CRT） during the delayed cardioprotection induced by hypoxic preconditioning （HPC） and the intracellular signal transduction mechanisms of the alteration. （1） Wistar rats wore randomly divided into three groups： sham operation group （Sham）, myocardial infarction （MI） group induced by left coronary artery ligation and HPC+MI group （4-hour HPC 24 h before MI）. Twenty-four hours, 14 d and 28 d after left coronary artery ligation, myocardial function, infarction size and the area at risk were measured. Western blot was used to detect the expression of CRT, the activity of p38 mitogen-activated protein kinase （MAPK） and stress-aedvated protein kinase （SAPK）. （2） Cultured cardiomyocytes from neonatal Sprague-Dawley （SD） rat were divided into six groups： hypoxia/ reoxygenation （H/R）, HPC, HPC+H/R, p38 MAPK inhibitor SB203580+HPC+H/R （SB+HPC+H/R）, SAPK inhibitor SP600125+HPC+H/R （SP+HPC+H/R） and control. Survival rate and apoptosis rate of cardiomyocytes 6 h after H/R and activities of lactate dehydrogenase （LDH） in culture medium in each group were measured. Western blot was used to detect the expression of CRT and activities of p38 MAPK and SAPK. The results are as follows： （1） During in vivo experiment, compared with MI group, HPC significantly improved ±dp/dt_(max) and -dp/dt_(max), reduced infarction size and the area at risk. HPC dramatically changed the expression of CRT. CRT expression in HPC+MI group was 206% of that in MI group （P〈0.05） 24 h after infarction, especially in the area at risk. However, 28 d after operation, the expression of CRT decreased by 57%. Correlation analysis indicated a positive correlation between CRT expression and myocardial function （r = 0.9867, P〈0.05）, and negative correlation between CRT expression and infarction size （r = -0.9709, P〈0.05）. （2） In cultured cardiomyocytes, HPC attenuated cell injury induced by H/R. CRT expression increased moderately to 222% of control （P〈0.05） during HPC, but increased dramatically to 503% of control （P〈0.05） after H/R. HPC reduced H/R-induced CRT up-regulation to 56% of that in H/R group （P〈0.05）. Correlation analysis indicated that CRT expression induced by HPC had a positive correlation with p38 MAPK activity （r = 0.9021, P〈0.05）, but a negative correlation with SAPK activity （r = -0.8211, P〈 0.05）. Both in vivo and in vitro results indicate that HPC protects myocardium from ischemia or H/R injury, p38 MAPK is possibly involved in the up-regulation of CRT induced by HPC, while SAPK has a negative influence.

Key words: calreticulin;Hypoxia;ischemic preconditioning;Mitogen-activated protein kinase

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Citing This Article：

Xu Feifei, Fu Yan, Liu Fengying, Zhu Xiaomei, Liu Xiuhua. Calreticulin expression increases during delayed cardioprotection induced by hypoxic preconditioning. Acta Physiol Sin 2006; 58 (6): (in Chinese with English abstract).