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Generation and characterization of antibody against paf1 complex in {sl Drosophila melanogaster}

Wei Wenxiang, Yang Jicheng, Zhuang Wenzhuo, Bai Yanyan, Sheng Weihua, Miao Jingcheng

Department of Cellular and Molecular Biology, School of Medicine, Soochow University.Suzhou 215123,Jiangsu

Abstract

Paf1 complex was identified in yeast and characterized to function in transcription and its related events. We identified the Drosophila homological components of pafl, CDC73 and RTF1 of paf1 complex. The genes encoding Drosophila pafl, CDC73 and RTF1 were cloned and expressed. With the purified recombinant proteins of truncated components of paf1 complex, antibodies against the Drosophila pafl, CDC73 and RTF1 were generated. These antibodies have been shown to be able to detect the endogenous pafl subunits as well as their human counterparts in the HeLa extract. On Drosophila polytene chromosomes, these antibodies have been demonstrated to locate the paf1 complex at actively transcribing sites, which co-localized with phosphorylated RNA polymerase Ⅱ, indicating that paf1 complex in Drosophila is involved in transcription or the events coupling with transcription.

Key words: pafl complex;paf1;CDC73;RTF1;transcription

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Citing This Article：

Wei Wenxiang, Yang Jicheng, Zhuang Wenzhuo, Bai Yanyan, Sheng Weihua, Miao Jingcheng. Generation and characterization of antibody against paf1 complex in {sl Drosophila melanogaster}. Acta Physiol Sin 2006; 58 (6): (in Chinese with English abstract).