Issue Archive

Inflammation of different tissues in spontaneously hypertensive rats

Sun Li, Gao Yuehong, Tian Dengke, Zheng Jianpu, Zhu Chunyun, Ke Yan, Bian Ka

Murad research institute for modernized Chinese medicine, Shanghai University of Traditional Chinese Medicine.Shanghai 201203

Abstract

The hypertension is one of chronic vascular diseases, which often implicates multiple tissues causing stroke, cardiac hypertrophy, and renal failure. A growing body of evidence suggests that inflammatory mechanisms are important participants in the pathophysiology of hypertension. In this study, the inflammatory status of these tissues (kidney, liver, heart, and brain) in spontaneously hypertensive rats (SHR) was analyzed and its molecular mechanism was explored. The tissues were dissected from SHR and agematched control Wistar-Kyoto (WKY) rats to investigate the abundance of inflammation-related mediators (IL-1#beta#, TNF#alpha#, ICAM-1,iNOS, C/EBP#delta# and PPAR#gamma#). mRNA levels were determined by reverse transcription-polymerase chain reaction and protein expression was evaluated by Western blot. To evaluate the oxidative stress of tissues, carbonyl protein content and total antioxidant capacity of tissues were detected by spectrophotometry and ferric reduction ability power (FRAP) method. The results suggest that: (1)Expressions of inflammation-related mediators (IL-1#beta#, TNF#alpha#, ICAM-1, iNOS, C/EBP#delta# and PPAR#gamma#) in SHR were higher compared with those in WKY rats except no evident increase of IL-1#beta# mRNA in liver and brain in SHR. (2) Tissues in SHR contained obviously increased carbonyl protein (nmol/mg protein) compared to that in WKY rats (8.93±1.08 vs 2.27±0.43 for kidney, 2.23±0.23 vs 0.17±0.02 for heart, 13.42± 1.10 vs 5.72± 1.01 for brain, respectively, P＜0.05). However, no evident difference in the amount of carbonyl protein in liver was detected between SHR and WKY rats. (3) Total antioxidant capacities of kidney, liver, heart and brain were markedly lower in SHR than that in WKY rats (P＜0.05). Thus, the present data reveal a higher inflammatory status in the important tissues in SHR and indicate that inflammation might play a potential role in pathogencsis of hypertension and secondary organ complications.

Key words: Spontaneously hypertensive rat;Inflammation;kidney;liver;heart;brain

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Sun Li, Gao Yuehong, Tian Dengke, Zheng Jianpu, Zhu Chunyun, Ke Yan, Bian Ka. Inflammation of different tissues in spontaneously hypertensive rats. Acta Physiol Sin 2006; 58 (4): (in Chinese with English abstract).