Issue Archive

Effects of gastric ischemia--reperfusion on gastric mucosal cellular apoptosis and proliferation in rats

Qiao Weili, Wang Lin, Zhang Jianfu, Zhang Yongmei

Department of Physiology,Xuzhou Medical College.Xuzhou 221002,Jiangsu；China

Abstract

The effect of gastric ischemia-reperfusion (GI-R) on gastric mucosal cellular apoptosis and proliferation was investigated using histological, immunohistochemical methods in Sprague-Dawley rats. The GI-R model was established by clamping the celiac artery for 30 min and reperfusing for 0, 0.5, 1, 3, 6, 24, 48, 72 h, respectively. Mild gastric mucosal injury was induced by ischemia alone. However, the injury was worsened and reached a maximum at 1 h after reperfusion, almost simultaneous with that the gastric mucosal cellular apoptosis was increased and cellular proliferation was decreased in gastric mucosa. Then, gastric mucosal cells began to repair by increased gastric cellular proliferation, which achieved a maximum at 24 h after reperfusion. The mucosal lesions were repaired almost completely at about 72 h after reperfusion. These results indicate that the gastric mucosal injury after GI-R is mainly induced by reperfusion. The damaged gastric mucosa could initiate its repairing mechanism immediately through inhibiting cellular apoptosis and increasing the number of proliferative cells, which substitute the damaged cells gradually. The plerosis almost completes in three days after reperfusion showing a strong self-repair ability of gastric mucosa.

Key words: stomach;Ischemia-reperfusion;Apoptosis;cellular proliferation;Rats

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Qiao Weili, Wang Lin, Zhang Jianfu, Zhang Yongmei. Effects of gastric ischemia--reperfusion on gastric mucosal cellular apoptosis and proliferation in rats. Acta Physiol Sin 2006; 58 (3): (in Chinese with English abstract).