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Role of bcl-2 transcriptional regulation induced by calmodulin Ⅰin pressure overload rat hypertrophic hearts

Zhou Qi, Xiao Yingbin, Liu Jian, Wang Peiyong, Chen Lin, Zhong Qianjin, Wang Xuefeng

Department of Cardiovascular Surgery, Xinqiao Hospital.Chongqing 400037；China

Abstract

This study was designed to evaluate the role of bcl-2 transcriptional regulation induced by calmodulinⅠ(CaMⅠ) in pressure overload rat hypertrophic hearts. The model of hypertensive Sprague-Dawley rat was established by abdominal aortic constriction. The hearts were collected four weeks after abdominal aortic constriction. Velocity and isopyknic gradient centrifugation was employed to fractionate rat myocardial nuclei. Western blot analysis revealed a marked increase in phosphorylated cAMP response-element binding protein (pCREB) of cardiac hypertrophy group compared with that in control group (P<0.05), while the protein level of cAMP response-element binding protein (CREB) was constant (P>0.05). Immunohistochemistry results showed a significant increase of CaMⅠprotein in cardiac hypertrophy group relative to the control group (P<0.05). Nuclear run off transcription assay displayed a significant increase in bcl-2 mRNA treated with trifluoperazne compared with non-drug treatment (P<0.05).The results obtained suggest that the transcription of bcl-2 is possibly regulated by CaMⅠin hypertrophic rat hearts, and CREB phosphorylation seems to be a minor factor in bcl-2 transcriptional regulation.

Key words: Myocardial hypertrophy;Calmodulin;cAMP response-element binding protein;Apoptosis

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Citing This Article：

Zhou Qi, Xiao Yingbin, Liu Jian, Wang Peiyong, Chen Lin, Zhong Qianjin, Wang Xuefeng. Role of bcl-2 transcriptional regulation induced by calmodulin Ⅰin pressure overload rat hypertrophic hearts . Acta Physiol Sin 2005; 57 (6): (in Chinese with English abstract).