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Resveratrol reduces intracellular free calcium concentration in rat ventricular myocytes

Liu Zheng, Zhang Liping, Ma Huijie, Wang Chuan, Li Ming, Wang Qingshan

Department of Physiology, Institute of Basic Medical Sciences, Hebei Medical University.Shijiazhuang 050017,Hebei；China

Abstract

Resveratrol (trans-3,4’,5-trihydroxy stilbene), a phytoalexin found in grape skins and red wine, has been reported to have a wide range of biological and pharmacological properties. It has been speculated that resveratrol may have cardioprotective activity. The objective of our study was to investigate the effects of resveratrol on intracellular calcium concentratoin ([Ca~(2+)]_(i)) in rat ventricular myocytes. [Ca~(2+)]_(i) was detected by laser scanning confocal microscopy. The results showed that resveratrol (15--60 #mu#mol/L) reduced [Ca~(2+)]_(i) in normal and Ca~(2+)-free Tyrode's solution in a concentration-dependent manner. The effects of resveratrol on [Ca~(2+)]_(i) in normal Tyrode's solution was partially inhibited by pretreatment with sodium orthovanadate (Na_(3)VO_(4), 1.0 mmol/L, P<0.01), an inhibitor of protein tyrosine phosphatase, or L-type Ca~(2+) channel agonist Bay K8644 (10 #mu#mol/L, P<0.05), but could not be antagonized by NO synthase inhibitor L-NAME (1.0 mmol/L). Resveratrol also markedly inhibited the ryanodine-induced [Ca~(2+)]_(i) increase in Ca~(2+)-free Tyrode's solution (P<0.01). When Ca~(2+) waves were produced by increasing extracellular Ca~(2+) concentration from 1 to 10 mmol/L, resveratrol (60 #mu#mol/L) could reduce the velocity and duration of propagating waves, and block the propagating waves of elevated [Ca~(2+)]_(i). These results suggest that resveratrol may reduce the [Ca~(2+)]_(i) in isolated rat ventricular myocytes. The inhibition of voltage-dependent Ca~(2+) channel and tyrosine kinase, and alleviation of Ca~(2+) release from sarcoplasmic reticulum (SR) are possibly involved in the effects of resveratrol on rat ventricular myocytes. These findings could help explain the protective activity of resveratrol against cardiovascular disease.

Key words: resveratrol;Rat;Myocytes;Intracellular calcium;Ca~(2+) channel

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Citing This Article：

Liu Zheng, Zhang Liping, Ma Huijie, Wang Chuan, Li Ming, Wang Qingshan. Resveratrol reduces intracellular free calcium concentration in rat ventricular myocytes . Acta Physiol Sin 2005; 57 (5): (in Chinese with English abstract).