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Effects of resveratrol on delayed afterdepolarization and triggered activity induced by ouabain in guinea pig papillary muscles

Zhang Liping, Ma Huijie, Zhao Juan, Wang Qingshan

Department of Physiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017, Hebei, China

Abstract

The purpose of this study was to investigate the effects of resveratrol on delayed afterdepolarization (DAD) and triggered activity (TA) induced by ouabain in guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded using intracellular microelectrode technique. The results obtained are as follows: DAD and TA induced by ouabain (1 #mu#mol/L) were inhibited by pretreatment with resveratrol (30, 60, and 120 μmol/L) in a concentration-dependent manner; Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME, 1 mmol/L), a nitric oxide (NO) synthase inhibitor, failed to abolish the above effect of resveratrol (60 #mu#mol/L ); 5 #mu#mol/L 17#beta#-estradiol (E2) or 30 #mu#mol/L resveratrol had no effects on DAD and TA, however, resveratrol combined with E_(2) at the same doses exerted significant inhibitory effects on DAD and TA; Pretreatment with tamoxifen (TAM, 10 #mu#mol/L), an inhibitor of estrogen receptor, also did not blocked the effects of resveratrol (60 #mu#mol/L) on DAD and TA induced by ouabain. All these results indicated that resveratrol exerted an inhibitory effects on DAD and TA induced by ouabain, possibly by reducing calcium influx, which might not be mediated by NO and estrogen receptor. The antiarrhythmic effects of resveratrol may contribute to its cardioprotective action.

Key words: resveratrol;Delayed afterdepolarization;Papillary muscle;Phytoestrogen;Calcium current

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Citing This Article：

Zhang Liping, Ma Huijie, Zhao Juan, Wang Qingshan. Effects of resveratrol on delayed afterdepolarization and triggered activity induced by ouabain in guinea pig papillary muscles. Acta Physiol Sin 2005; 57 (3): (in Chinese with English abstract).