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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

A method of isolating P cells of rat sinoatrial node by collagenase is described. The method is feasible for morphological and electrophysiological investigation, with the advantages of taking a relatively easy procedure and consuming a small amount of collagenase. The main features of action Potentials of these isolated cells including clear diastolic automatic depolarization, mean maximal diastolic potential of -55 mV, mean peak potential value of 58 mV, and mean APD50 of 18 ms, were similar to those of P cells In situ.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).