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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

In the Present study,the effect of endotoxin on the radioimmununoassay of calcitonin gene-related peptide(CGRP)was investigated by method of radioimmunoassay. The results showed a competitve combination of endotoxin at high-concentrations (higher than 5μg/ml) with multiclonal antibody of CGRP, and the cross immunoreaction ratio between CGRP and endotoxin was 5 .6 ×10-6. The mixed endotoxin in samples was effectively cleared through C18 column purification. The data indicated that endotoxin interfered CGRP radioimmunoassay in vitro at high concentrations. The influence of endotoxin on CGRP edoimmunoassay can be avoided through C18 colunm Purification for the endotoxin-containing samples.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).