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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Cell growth and proliferation were evaluated in cultured vascular smooth muscle cells (VSMCs) isolated from spontaneously hypertensive rat (SHR) and normotensive Wistar-Kyoto (WKY) rat aortae by measuring [3H]-thymidine incorporation into newly synthesize DNA and by determining cell number, respectively. The results showed that in cultures from both rat strains(1) serum, basic fibroblast growth factor (bFGF)- and thrombin-induced DNA synthesis were inhibited by L-phenylanine dosedependently; (2) L-phenylalanine inhibited cell proliferation in response to serum in a concentration ependent manner; (3) L-phenylalanine inhibited serum-induced proto-oncogene c-fos and c-myc expression;(4)L-tyrosine, L-histidine and D-phenylalanine failed to minic the inhibitory effect of L-phenylalanine. All these data demonstrate that L-phenylalanine could exert a direct and specific antiproliferative effect on VSMCs suggesting that such effect can account for the antihypertensive action of this amino acid observed in SHR.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).