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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Seizure-sensitive Fisher 344 rats were prepared 7 d after seizure episodes induced by a single subcutaneous administration of convulsive dose (7 .5 mg/kg)of kainic acid. The activator protein-1 (AP1) DNA binding activity and its components in the hippcampus of such rats were examined by Gel shift,Super-shift and Western blot assay.Gel shift assay showed that the basal level of ET-1 im binding activity was significantly higher than that of control rat hippocampus. Super-shift showed that only Fra and Jun D antibodies could super-shift AP-1 complex to a higher position, It suggested that the AP-1 complex was composed of Fra and Jun D in hippocampus of the seizure-sensitive rats. Western blot analyais further confirmed that Jun D proteins contained components of 43,39 and 28 kDa. When secondary seizure stimulations were given to seizure-sensitive rate, the AP-1 DNA binding activity was attenuated and the components of AP-1proteins changed transiently.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).