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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

In this study, the fluorescent dye Fluo-3 (AM) was applied to rat hippocampus by dricroinjection, to label neurons in situ. A large number of CA1 pyramidal cells could be labeled by a single injection. With this improvement of labeling and the use of the confocal laser scanning miecroscope (Leica Lasertechnik GmbH), changes of calcium fluorescence intensity in CA1 pyrdridal cells of rat hippocampal slices on the models of mimic epilepsy (induced by penicillin or glutarnate) or hypoglycemia/hypokia were studied in vitro. The resultS showed that in magnesium free axtificial CSF, the intracellulax calcium level of tie CA1 ppeAndal cells increased following application of glutamate or penicillin. Singular phenomenon Was also observed on the in vitro model to hypoglycemia/hypoxia.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).