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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

The effects of protein kinase C (PKC) and protein kinase A (PKA) on the high affinity glutalnate uptake in rat cerebral cortex synaptosomesg cell lines of human neuroblastorna SK-N-SH and human anaplastic astrocytoma BT-325 were studied by measurement of radioactivity of 3 [H] -L-glutarnate. The results were as follows: (1)PKC agonist phorbol-12-myristate, 13-acetate (PMA) stimulated the glutamate uptake in foe rat cerebral cortex synaptosomes and cell line of BT-325, but not in the cell line of SK-N-SH. This effect of PMA was abolished in the presence of PKC antagonist sphingosine. (2) Glutarnate uptake in all these three samples of neural tissues and cells was not edicted by PKA agonist adenosine 3', 5 1 - cycli cmonopho sphate, N6, O2' dibutyryl. The above results indicate that it is PKC that stimulates high sanity glutamate uptake in glial cells, although the possibility that neurons are also affected to some extent can not be mied out.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).