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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Cell viability and cell membrane disruption of rat astrocyte after heat shock response (HSR) were assessed by the analysis of MTT reduction and lactate dehydrogenase (LDH)release. We studied whether HSR would modulate the susceptibility of astrocyte to H2O2induced oxidative stress. Cell viability was assessed by reduction of Mrs. HSR in 43℃water hath for 30 min decreased H2O2 toxicity (P<0.01) to astrocyte. HSR induced decrease in H2O2 (50μmo;/L) toxicity was also shown by the reduction in the release of LDH, which was a marker of cell melnhrane dismption. The result also showed that Prior to the incubation in 43℃ water bath for 30 min strongly increased IL-6 release 6 h (P <0. 05 ) after HSR. The above data suggest that the enhanced release of IL-6 from astrocyte may be one of the mechanisms underlying the cell protective effect induced by HSR.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).