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Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

The present study was undertaken to investigate whether GABA induced the acrosome reaction (AR) and fertilizing ability, as well as its POssible mode of action in human spermatozoa. Spermatozoa from fifteen health fertile men isolated by the swim-up techniqUe were preincubated in a modified Bac with 0.35% BSA for 1-11 h under 5% CO2 in 95% air at 37℃. Aliquots of spermatozoa were collected at 0, 1, 3, 5,7, 9 and 11 h of incubation for evaluation of foe AR by chlortetracycline (CTC)staining. The sperm penetration assay (SPA) was carried out by using the zona-free hamster oocyte test. Intracellulax calcium ([ Ca2+ ]i ) concentrations were detendned by means of fluorescent probe Frua-2/AM. GABA at 1 .25 Umol/L significantly induced the AR in human spermatozoa preincubated for 3 h, with a maximal response in preincubated for 9 h, and the effect changed in a concentration- dependent manner.

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Citing This Article：

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).