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Changes of k~(+) channel current in cerebellar cortical neuron of apoptosis induced with H_(2)0_(2)

Chi Xianxuan, Feng Jianqiang, Chen Peixi

Department of Physiology，Sun Yat-Sen University of Medical Science. Guangzhou 510089,China

Abstract

The neurons dissociated from the cerebellar cortex of neonatal SD rat were cultured,while the growth of non-neuron cells were suppressed hy Ara-c. Apoptosis of the neurons were induced with H_(2)O_(2). The changes of I_(k) were studied during apoptosis using the patch clamp technique in cell-attached confgurations. The results are as follows: (1) the Ik amplitudes of the neurons were lower than that of normal at various corresponding clamp voltages; (2) the conductances, mean open time,open probability, long and short opening time constants were all lower, as compared with normal neurons. All these results indicate that the activities of K~(+) channels on cerebellar neurons decrease during apoptosis.

Key words: Cerebellar cortical neuron;Apoptosis;H_(2)O_(2)

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Citing This Article：

Chi Xianxuan, Feng Jianqiang, Chen Peixi. Changes of k~(+) channel current in cerebellar cortical neuron of apoptosis induced with H_(2)0_(2). Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).