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Inhibition of the expression of #delta# opioid receptor mRNA BY Long-term exposure of NG-108-15 cell line to #delta# opioid agonists

Wang Yun, Wang Xiaomin, Han Jisheng

Neuroscience Research Center，Beijing Medical University. Beijing 100083,China

Abstract

We used the method of reverse transeription-polymerase chain reaction (RT-PCR) to observe the effects of long-term adrinistration of peptide a opioid receptor agonist DPDPE and non-peptide #delta# agonist BW 73U86 on the mRNA expression of #delta# receptor and the possible difference between the two agonists.The results showed: (1) the level of #delta# receptor mRNA decreased significantly 24-48 h after administration of DPDPE; the effect of BW373U86 lasted only 24 h; and (2) DPDPE produced a signiticant decrease of δ-receptor mRNA at 10~(-6)mol/L, Whereas BW373U86 was effective only at 10~(-5)mol/L,These results suggest that long-term administration of both peptide #delta# opioid receptor agonist (24, 48 h) and non-peptide #delta# opioid agonist (24 h) significantly decreased mRNA expression of δ opioid receptor and the effect of peptide agonist DPDPE seems to be stronger and last longer than that of non-peptide agonist BW373U86.

Key words: D-Pen~（2 5）] enkephalin;BW373U86;#delta# opioid receptor;mRNA;Down-regulation reverse transcription-polyrnerase chain leaction;

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Citing This Article：

Wang Yun, Wang Xiaomin, Han Jisheng. Inhibition of the expression of #delta# opioid receptor mRNA BY Long-term exposure of NG-108-15 cell line to #delta# opioid agonists. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).