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Effect of Arg-Gly-Asp-Ser(RGDS) peptide on adp-induced signal transduction of activated rat platelet

Yao Ringhai, Wang Peiyong, Pang Yongzheng, Su Jingyi, Tang Chashu

Institute of Cardiovascular Basic Research Beijing Medical University. Beijing 100083,China

Abstract

The purpose of this work was to investigate the effect of Arg-Gly-Asp-Ser (RGDS)peptide on platelet aggregation, protein phosphorylation, protein kinase C (PKC) and thetogen-activated protein kinase (MAPK) activity during platelet achvation. Experiments were performed on ADP activated rat platelets in vivo.Results showed that 50 #mu#mol/LADP, in addition to inducing platelet aggregation, obviously enhanced not only PKC and MAPK activities but also 95 and 66 kD protein phosphorylation. When platelets and ADP were incubated t0gether with 50, 100,200 #mu#mol/L RGDS peptide it was found that the latter markedly inhihited ADP activated platelet aggregation and activation of PKC and MANK, both in a concentration-dependenily manner.RGDS peptide also inhibited 95 and 66 kD protein phosphorylation concentration-dependently and went positively with its activation of PKC and MAPK.

Key words: RGDS peptide;Platelet aggregation;Protein phosphorylation;Protein kinase C; Mitogenactivated protein kinase;Signal transduction

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Citing This Article：

Yao Ringhai, Wang Peiyong, Pang Yongzheng, Su Jingyi, Tang Chashu. Effect of Arg-Gly-Asp-Ser(RGDS) peptide on adp-induced signal transduction of activated rat platelet. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).