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The relationship between cytosolic Ca~(2+) and two kinds of granule number in activated platelets

Ding Jean, Yu Zhang, Rong Damei, Zhong Cisheng

Department of Biophysics，Shanghai Medical University. Shanghai 200032,China

Abstract

The numerical density (Nv) of the #alpha#-granule (#alpha#G) and dense grantile (dG)measured by using electron dricroscopic morphometry and the cytosolic free calcium concentration ([Ca~(2+)]_(i)) by using Ca-fluorescent indicator were observed for studying the relationship of [Ca~(2+)]_(i) and Nv of αG and dG in platelets activated by A23187, thrombin or ADP. The results showed that the [Ca~(2+)]_(i) rose markedly by A23187. Thrombin or ANP could also induce an increase of [Ca~(2+)]_(i) concentration-dependently. Under 3 different degrees of activation induced by different agonists,the close relationship between Nv of both granules, #alpha#G and dG, and [Ca~(2+)]_(i) in platelets was found (P_(r(#alpha#G))< 0.05,P_(r(dG))<0.01). These findings indicate that the increase in [Ca~(2+)]_(i) may enhance the secretion of #alpha#G and dG.

Key words: Platelet;#alpha#-granule;Dense granle;Cytosolic Ca~(2+);Fura2

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Citing This Article：

Ding Jean, Yu Zhang, Rong Damei, Zhong Cisheng. The relationship between cytosolic Ca~(2+) and two kinds of granule number in activated platelets. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).