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Properties of the gm-csf-induced outward K~(+) current in niurine peritoneal exudate macrophages

Zhang Xiaofeng, Feng Meifu, Wu Caihong, Zhou Peiai

National Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology，Chinese Academy of Sciences. Beijing 100080,China；College of Life Sciences，Peking University.Beijing 100871,China

Abstract

Whole-cell patch-clamp technique was used to study the changes of ionic currents in murine peritoneal exudate macrophages (PEMs ) prestimulated with granulocytemacrophage colony-stimulating factor (GM-CSF,16ng/ml) for periods from 0.5 up to 6 d. The GM-CSF-treated PEMs developed a GM-CSF-induced transient inactivating outwwh K~(+) current (I_(A)). IA showed steady-state inactivation over the physiological voltage range and possessed frequency dependence of inactivation When depolarizing pulses were applied at a frequency of 0.5 Hz.IA was selectively inhibited by extracellulax 4-AP (3 mmol/L). When [Ca~(2+)], was increased (from pCa 8 to 6), the amplitudes of IA were depressed significantly. When the PEMs were exposed to cycloheximide (0.3#mu#g/ml), a protein synthesis inhibitor, for 12 h, I_(A) expression was completely suppressed. It was notable that the changes of the current expression, activation hehavior and kinetic properties occurred during GM-CSF treatment.

Key words: Graselocyte-macrophage colony-stimulating factor;Outward K~(+) current; Macrophage;Patch-clamp

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Citing This Article：

Zhang Xiaofeng, Feng Meifu, Wu Caihong, Zhou Peiai. Properties of the gm-csf-induced outward K~(+) current in niurine peritoneal exudate macrophages. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).