Issue Archive

MA Yong-Jie

2College of Information Engineering, Taishan Medical University, Taian 271016, China

Abstract

Endothelin-1(ET-1)has been shown to be a potent growth factor and to induce cardiac hypertrophy.In the present study,we examined the role of G protein,protein kinase C(PKC)and Na+ -H+ exchanger in ET-1-induced cardiac hypertrophy in cultured neonatal rat cardiac myocytes.ET-1(1O-10～10-7 mol/L)induced promotion of 3H leucine incorporation,increase in cell protein contnt and cell surface area in a dose dependent manner with EC50 value of 5.2×1O-10,5.2×1O-10 and7.3×10-10 mol/L respectively.All of these ET-1-induced cardiomyocyte hypertrocphic responses were completely blocked by pretreatment with staurosporine(2 nmol/L),a protein kinase C inhibitor,and stimulated by 4-phorbol,12-myristate,13-acetate(PMA)(10-8～1O-6 mol/L),a protein kinase C artivator,in a dose-dependent manner.Pretreatment of amiloride(10-4 mol/L),a Na+ -H+ exchang inhibitor completely inhibited the ET-1 induced,but not PMA-induced cardiomyocyte hypertrophic responses.

Key words: ;;;;;

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

MA Yong-Jie. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).