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Li Wenjue

Department of Physiology, Shanghai College of Traditional Chinese Medicine. Shanghai, China

Abstract

This study was designed to investigate whether prolactin(PRL)affects coordinated regulation of tissue plesminogen activator(tPA)and plasminogen activator inhibitor type-I(PAI-I)gene exprssion in rat granulosa cells in vitro.Several methods,such as SDS-PAGE,immunoblot etc.were used to detect the effect of PRL on tPA and PAI-I gene expression.The results demonstrated:(1)PRL increases PaI-I mRNA production in cultured granulosa cells.Inclusion of follicle stimulating hormon (FSH) with PRL has a synergistic effect on the increase of PAI-I mRNA levels.After 48 h culture in the presence of FSH with PRL,a 7.8-fold increase in PAI-I mRNA levels is observed as compared with PRL alone.The synergistic increase in PAI-I MRNA levels occurs in a dose-and time-dependent manner;(2)the increase in PAI-I mRNA synthesis in the cells by PRL alone,or PRL in combination with FSH,is well correlated with the changes of PAI-I activity levels in the conditioned media.

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Citing This Article：

Li Wenjue. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).