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Effects of penicillin and phenobarbital on the ~(3)H-GABA accumulation in slices of mice brain

Xu Xianggui, Zhu Jianqin, Wang Xinguang, Wang Fongping

Section of Physiology, Department of Biology, Nanjing University

Abstract

The effects of convulsant potassuim benzylpenicillin (PG) and anticonvulsant
sodium phenobarbital (PhB) on the ~(3)H-GABA accumulation in the slices of whole
mice brain were studied in vivo and vitro. The results were as follows: (1) 100#mu#1 PG 6.7-13.40×10~(-4)mol/L or 50#mu#l PhB 19.60-39.20×10~(-4)mol/L in their incubative medium (2m1) caused二marked decrease of accumulation of ~(3)H-GABA in the brain slices (P<0.05) ,when PG 6.70×10~(-4)mol/L, 100#mu#l was added together with PhB 39.60×10~(-4)mol/L, 50#mu#l into the incubative medium (2ml), the ~(3)H-GABA accumulation in brain slices was somewhat higher than the individual PG in vitro. (2) Intracerebroventricular(ICV)injection of 20#mu#IPG 3.35×10~(-2)mol/L resulted in a potent convulsant activity in mice, with a decrease of ~(3)H-GABA accumulation in the brain slices. In contrast, ICV injection of 10#mu#l PhB 3.88×10~(-2)mol/L caused an anticonvulsant action, also with a decrease of ~(3)H-GA-BA accumulation. On the other hand, when PG and PhB were simultaneusly administered, the accumulation of ~(3)H-GABA in the brain slices recovered to the normal level. The above results prove that the convulsant of PG and the anticonvulsant of PhB are related to GABA accumulation in brain and synaptic process of GABA.

Key words: Convulsant;Anticonvulsant;Penicillin;Sodium phenobarbital;Whole brain slices;~(3)H-GABA;Accumulation

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Citing This Article：

Xu Xianggui, Zhu Jianqin, Wang Xinguang, Wang Fongping. Effects of penicillin and phenobarbital on the ~(3)H-GABA accumulation in slices of mice brain. Acta Physiol Sin 1987; 39 (5): (in Chinese with English abstract).