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Nestin mRNA expression during the development on mouse central nervous system

Yang Jing, Bian Wei, Jing Naihe

Shanghai Institute of Biochemistry, Chinese Academy of Science. Shanghai 200031 China

Abstract

Intermediate filament Nestin is a marker of neural precursor cells. Its expression pattern during mouse brain development was studied in the present investigation. A pair of primers based on the sequence of mouse Nestin were found best to be suit for the reverse transcriptional PCR (RT-PCR) with this pair of Nestin Primer. Using RT-PCR, Nestin transcripts were detected during the development of the central nervous system (CNS) of mouse, from embryonic day 10 to adult brain. We found that the Nestin showed a transient expression in the development of cerebrum. At the embryonic day 14, the expression of Nestin mRNA reached the highest peak, and was then down-regulated. In the postnatal cerebellum, Nestin gene was also expressed in a transient pattern. The highest peak of Nestin mRNA expression emerged at postnatal day 5, and was then down-regulated. Nestin mRNA expression in 10 kinds of adult mouse tissues did not yield any positive results.

Key words: Nestin gene;Reverse transcriptional PCR;mRNA expression;Neural development

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Citing This Article：

Yang Jing, Bian Wei, Jing Naihe. Nestin mRNA expression during the development on mouse central nervous system. Acta Physiol Sin 1997; 49 (6): (in Chinese with English abstract).