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The synthetic fragment of rabbit blastocyst peptide_(2) and its physiological significance relevant to the mechanism of implantation

Cao Yongqing, Chen Youzhen, Zhang Fuchun

State Key Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica. Beijing 100080, China

Abstract

The amino acid sequence of purified rahbit blastoeyst peptide_(2) (RBP_(2)) was analyzed. It contains 54 amino acids and it's sequence from aa4 to aa34 is the same as the N-terminal sequence of uteroglobin. An active antigenic region consisting of l5 aa residues (aa20-aa34) of the RBP_(2) was selected and designated as a synthetic peptide fragInent (SPF). The effect of SPF on lymphocyte proliferation was analyzed by immunocytochemical assay detecting BrdU incorporation in the cellular DNA. The results indicated that no obvious inhibitory effect of SPF on T cell proliferation at concentrations ranging from 80 to 320 #mu#g/ml was observed, while RBP_(2) at the concentrations ranging from 20 to 160 #mu#g/ml had a significant effect in a dose dependent manner, suggesting that the immuno-suppressive effect of SPF is less than that of RBP_(2). We have also observed that SPF at concentrations ranging from 45 to 180 #mu#g/ml inhibited PGF_(2#alpha#) secretion in a dose dependent manner.

Key words: Implantation;Rabbit blastocyst peptide;Synthetic peptide fragment

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Citing This Article：

Cao Yongqing, Chen Youzhen, Zhang Fuchun. The synthetic fragment of rabbit blastocyst peptide_(2) and its physiological significance relevant to the mechanism of implantation. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).