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In vitro stimulation of HTC hepatoma cell growth by recombinant human augmenter of liver regeneration (ALR)

Yang Xiaoming, Hu Zhiyuan, Xie Ling, Wu Zuze, Wu Chutse

Beijing Institute of Radiation Medicine. Beijing 100850, China

Abstract

It was demonstrated that biologically active ALR could be expressed from its cDNA in transient expression experiments in cos-7 cells. The results showed that the cytosolic fraction from the transfected cells with constructed plasmids DNA stimulated of DNA synthesis of in vitro HTC cells in a dose dependent manner. This finding'suggests that the grC hepatoma cell line may be used as a target for bioassay of human ALR in vitro.

Key words: Liver regeneraion;Augmenter of liver regeneration;Expression;DNA synthesis

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Citing This Article：

Yang Xiaoming, Hu Zhiyuan, Xie Ling, Wu Zuze, Wu Chutse. In vitro stimulation of HTC hepatoma cell growth by recombinant human augmenter of liver regeneration (ALR). Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).