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Rapid and sensitive assay of monoamine iransmitters and their metabolites in biological samples using high performance liquid chromatography with electrochemical detection

Ye Weiling

Shanghai Institute of Physiology, Academy Sinica

Abstract

In the present experiment, monoamine transmitters and their metabolites, which had very analogous structure, were simultaneously separated and measured by reversed-phase ion-pair chromatography with electrochemical detection. The
isocratic elution was used and this method was able to determine nine compounds
among these compounds, at least, at one run during 30 min. The preparation of
biologic samples was simple, so the mean recovery could reach over 9000. The
mean coefficient of variation in retention time of ten compounds (include 3,4-dihydroxybenzylamine) was 0.73%±0.18% (mean1 S.D.), and the mean coefficient
of variation in peak area was 1.11%±0.45% (mean1 S.D.). In the range of 50-100pg to 40ng, the linear relation between the amounts and the peak areas was
accurate (r=0.996±0.006, mean±S.D.). The chromatographic conditions performed in the present assay were discussed.

Key words: Reversed-phase ion-pair chromatography;Elecctrochemical detection;Isocratic elution;D-camphor-10-sulfonic acid;Monoamine transmitters;Metabolites

Received: 　　Accepted:

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Citing This Article：

Ye Weiling. Rapid and sensitive assay of monoamine iransmitters and their metabolites in biological samples using high performance liquid chromatography with electrochemical detection. Acta Physiol Sin 1987; 39 (4): (in Chinese with English abstract).