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Isolation and identification of C_(12-h) peptide from the skin of Bombina Orientalis in China

Chen Feng, Zhao Yiqian

Department of physiology, Tong-Ji Medical University

Abstract

caused contraction of the smooth muscles of rat in vitro. The crude extracts were isolated by column chromatography of alkaline alumina, and a bioactive fraction was obtained from the 80%ethanol eluate. Submitting the fraction on Sephadex G_(-12) column.we found that the early eluate C_(-12),showing more bioactivity, was inactivated by hydrolysis with chymotrypsin, but not completely antagonized by cyproheptadine. C_(-12) was further analyzed by reversed phase-high performance liquid chromatography (RP-HPLC).The elution time of C_(-12-h) peak was the same as that of bombesin, and these two peptides had the same amino acid composition. These experimental results suggest that C_(-12-h) peptide is most probably bombesin.

Key words: Bombina orientalis;C_(-12-h) peptide;Bombesin

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Citing This Article：

Chen Feng, Zhao Yiqian. Isolation and identification of C_(12-h) peptide from the skin of Bombina Orientalis in China. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).