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Isolation of cardiomyocytes from the adult mouse heart

Zhang Ran, Yu Zhibin, Wang Yunying

Department of Aerospace Physiology, Fourth Military Medical University. Xi'an 710032, China

Abstract

In order to culture cardiomyocytes or to observe the contractile function of adult mouse cardiomyocytes, it is necessary to isolate high-yield and high-quality cardiomyocytes at first. The mouse was injected with heparin (5 000 IU/kg, i.p.) 20 min prior to the experimental protocol, then was sacrificed by cervical dislocation. The heart was excised and the aorta was cannulated rapidly. The cannulated heart was mounted on a Langendorff perfusion apparatus with constant flow and perfusion pressure was monitored. The initial perfusion pressure was maintained at 40 mmHg by regulating the flow rate. The heart was digested by 0.05 % crude collagenase I at 37 oC and the enzymatic digestion was terminated immediately when the perfusion pressure was lowered to 28 mmHg.

Key words: Mice;Cardiac myocytes;Isolation

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Citing This Article：

Zhang Ran, Yu Zhibin, Wang Yunying. Isolation of cardiomyocytes from the adult mouse heart. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).