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Stimulatory effects of gonadotropin-releasing hormone and dopamine on growth hormone release and growth hormone mRNA expression in Epinephelus coioides

Ran Xueqin, Li Wensheng, Lin Haoran

Institute of Aquatic Economic Aninals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Zhonghsan University. Guangzhou 510275, China；School of Animal Science, Guizhou University. Guiyang 550025, China

Abstract

Gonadotropin-releasing hormone (GnRH) and dopamine (DA) can stimulate growth hormone (GH) release, but their effects on GH mRNA synthesis are controversial and deficient in fish. Orange-spotted grouper (Epinephelus coioides) is a hermaphroditic marine fish with sex reversal. Few data are available concerning the regulation of GH in grouper. In the present study, the effects of GnRH and DA on GH release and GH mRNA expression were determined using pituitary fragments of orange-spotted grouper under static culture conditions. After incubation from 1 h to 24 h, salmon GnRH (sGnRH, 100 nmol/L) stimulated the release of GH and increased the level of GH mRNA time-dependently.

Key words: Growth hormone (GH);GH mRNA;Static incubation;Gonadotropin-releasing hormone (GnRH);Dopamine (DA);Apomorphine(APO);

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Citing This Article：

Ran Xueqin, Li Wensheng, Lin Haoran. Stimulatory effects of gonadotropin-releasing hormone and dopamine on growth hormone release and growth hormone mRNA expression in Epinephelus coioides. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).