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Biochemical and immunofluorescent identification of the Tubulin in Nervous system of shrimp (Penaeus Orientalis)

Jiang Mingshi, Zhao Weiqin, Sun Liqung, Hsu Ke

Shanghai Institute of Physiology, Chinese Academy Sinica

Abstract

The tubulin in the nervous system of shrimp (Penaeus orientalis) was purified
according to the method of ShelanskiEb3, which can be dissociated by SDS into two subunits of similar MW (about 55,000) and designated as #alpha# and #beta#. Using the purified tubulin from chicken brain as antigen we prepared the antisera from rabbits. With fluorescence-labeled goat anti-rabbit IgG the distribution of the tubulin in nerve fibers of shrimp was observed. It is interesting to find, besides the microtubular sheath and axoplasma, the myelin sheath in the myelinated nerve fibres of shrimp also showed rather strong fluorescent reaction. This means that the myelin sheath contained significant amount of tubulin which have not been seen in myclin sheath of other animals.

Key words: Microtubule;Tubulin;Nerve fiber of shrimp;Myelin sheath

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Citing This Article：

Jiang Mingshi, Zhao Weiqin, Sun Liqung, Hsu Ke. Biochemical and immunofluorescent identification of the Tubulin in Nervous system of shrimp (Penaeus Orientalis). Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).