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Volume-activated Cl- current in migrated nasopharyngeal carcinoma cells

Qian Lingjia

Institute of Hygiene and environmental Medicine, Academy of Military Medical Sciences. Tianjin 300050, China

Abstract

The transwell chamber migration assay and the patch-clamp technique were used to investigate the volume-activated Cl- current (ICl.vol) in migrated nasopharyngeal carcinoma cells (CNE-2Z). 47％ hypotonic solution activated a ICl.vol in the migrated CNE-2Z cells.Compared with the control cells (non-migrated), the properties of this current and the sensitivity to Cl- channel blockers were changed.The current density in migrated CNE-2Z cells was higher than that in non-migrated cells. The current was almost completely inhibited by extracellular application of adenosine-5'-triphosphate (ATP, 10 mmol/L), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB, 100mmol/L) and tamoxifen (30 mmol/L) in all voltage steps applied. The inhibition of NPPB and tamoxifen on the current was stronger in migrated cells than that in non-migrated cells. The permeability sequence of the four anions was Br-＞Cl-＞I-＞Gluconate. The sequence was different from that of the non-migrated cells (I-＞ Br-＞Cl-＞Gluconate). The results suggest that volume-activated chloride channels may be involved in the CNE-2Z cell migration.

Key words: Tumor cells;Cultured;Chloride channels;Cell migration;Patch-clamp techniques

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Citing This Article：

Qian Lingjia. Volume-activated Cl- current in migrated nasopharyngeal carcinoma cells. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).