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Nuclear factor kappa B signal transduction in macrophages during hypoxia: reactive oxygen species generation

Zhang Cuiping, Xie Yinzhi, Chen Peng, Hong Xin, Xiao Zhonghai, Ma Yan, Lu Yongda

Institute of Health and Environmental Medicine, Academy of Military Medical Sciences. Tianjin 300050, China；Institute of Radioactive Medicine, Academy of Military Medical Sciences. Beijing 100850, China

Abstract

The effects of hypoxia on the level of reactive oxygen species (ROS), IκBα tyrosine phosphorylation, transcription of P65 mRNA and NF-κB activation in isolated rat peritoneal macrophages were investigated by DCFH-DA fluorescence spectrophotometry, Western blotting and RT-PCR. The results obtained are as follows. (1) During hypoxia, the levels of intracellular ROS began to increase at 1 h, then reached a peak at 2 h, and began to decrease after 3 h. IκB#alpha# tyrosine phosphorylation began to rise after 2 h hypoxia and was the highest after 3 h hypoxia. After 4 h hypoxia it decreased gradually. NF-κB activation began to increase after 3 h hypoxia, and reached a peak after 4 h hypoxia. (2) When antioxidant NAC (500 #mu#mol/L) was added into the medium, the level of IκB#alpha# phosphorylation showed no significant changes during hypoxia. After adding protein tyrosine kinase inhibitor genistein (200 #mu#mol/L), NF-κB activation induced by hypoxia was blocked significantly.

Key words: Hypoxia;Macrophages;Reactive oxygen species;Nuclear factor kappa B

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Citing This Article：

Zhang Cuiping, Xie Yinzhi, Chen Peng, Hong Xin, Xiao Zhonghai, Ma Yan, Lu Yongda. Nuclear factor kappa B signal transduction in macrophages during hypoxia: reactive oxygen species generation. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).