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Effect of genistein on L-type calcium current in guinea pig ventricular myocytes

Han Taizhen

Department of Physiology, School of Medicine, Xi'an Jiaotong University. Xi'an 710061, Shanxi, China

Abstract

This paper was aimed to study the effect of genistein (GST) on L-type calcium current (ICa,L)in isolated guinea pig ventricular myocytes using whole cell patch-clamp recording technique. The results are as follows. (1) GST (10, 50, 100 μmol/L) reduced the voltageactivated peak amplitude of ICa,L in a concentration-dependent manner. Daidzein (100 μrnol/L), a structural analogue of GST which has little or no inhibitory effect on tyrosine kinase, produced no effect over the same concentration range on ICa,L (n=5, P＞0.05). (2) GST up- shifted the current-voltage (I-V) curve, but the characteristics of I-V relationship were not significantly altered, and the maximal activation voltage of ICa, L was not different from that of control. GST did not affect the activation kinetics of ICa, L.

Key words: Genistein;Patch-clamp techniques;Myocardium;L-type calcium channels

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Citing This Article：

Han Taizhen. Effect of genistein on L-type calcium current in guinea pig ventricular myocytes. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).