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Limb ischemic preconditioning attenuates apoptosis of pyramidal neurons in the CA1 hippocampus induced by cerebral ischemia-reperfusion in rats

Zhao Honggang, Li Wenbin, Li Qingjun, Chen Xiaoling, Liu Huiqing, Feng Rongfang, Ai Jie

Department of Pathophysiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017, China

Abstract

The purpose of this study was to investigate the effects of limb ischemic preconditioning (LIP) on apoptosis of pyramidal neurons in the CA 1 hippocampus induced by global cerebral ischemia-reperfusion in rats. Forty-six rats whose bilateral vertebral arteries were occluded permanently were assigned to one of four groups: sham group, limb ischemia group, cerebral ischemia group and LIP group. LIP was performed by occluding the bilateral femoral arteries for 10 min 3 times in an interval of 10 min. Global cerebral ischemia was underwent by occluding the bilateral common carotid arteries for 8 min immediately after LIP. Assays for apoptosis of the hippocampal neurons were biologically and morphologically performed using gel electrophoresis, TUNEL and AO/EB staining. Characteristic DNA ladder was clearly visualized with gel electrophoresis in the hippocampus in cerebral ischemia group, but not in LIP group.

Key words: Pathophysiology;Limb ischemic preconditioning;Cerebral ischemia;Apoptosis;Hippocampus;Rat

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Citing This Article：

Zhao Honggang, Li Wenbin, Li Qingjun, Chen Xiaoling, Liu Huiqing, Feng Rongfang, Ai Jie. Limb ischemic preconditioning attenuates apoptosis of pyramidal neurons in the CA1 hippocampus induced by cerebral ischemia-reperfusion in rats. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).