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Myocardioprotective effects of the combination of ischemic preconditioning with hypothermia and crystalloid cardioplegia in immature rabbits

Zhang Zhongming, Zhang Yucai, Xu Pengxheng, Dong Hongyan, Fan Jianwei, Zeng Yinming

Jiangsu Institute of Anesthesiology, Xuzhou Medical College, Department of Anesthesiology, The Third Hospital of Xuzhou City. Xuzhou 221002；Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College. Xuzhou 221002, China

Abstract

This study was undertaken to explore the myocardioprotective effects of the combination of ischemic preconditioning (IP) with hypothermia and St. Ⅱ Thomas crystalloid cardioplegic solution (CCS) on immature hearts in the rabbit. Isolated immature rabbit hearts were perfused with Krebs-Henseleit bicarbonate buffer on Langendorff apparatus. In experiment 1, 24 hearts were divided into 4 groups (n=6 in each group): Con, IP1, IP2 and IP3 group. Hearts of the four groups underwent 0, 1, 2 or 3 cycles of IP respectively. Then all the hearts were subjected to a sustained ischemia period of 2 h at 20℃ and a postischemic reperfusion period of 30 min at 37℃. In experiment 2, 48 hearts were divided into 6 groups (n=8 in each group): SConl, SIP1, SCon2, SIP2, SCon3 and SIP3 group, according to hypothermia and the duration of sustained ischemia (30 min at 32℃; 90 min at 25℃, 2 h at 20℃). The SIP1, SIP2 and SIP3 groups were preconditioned twice before the sustained hypothermic ischemia, while the SConl, SCon2 and SCon3 groups were not preconditioned.

Key words: Ischemic preconditioning;Myocardium;Hypothermia;

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Citing This Article：

Zhang Zhongming, Zhang Yucai, Xu Pengxheng, Dong Hongyan, Fan Jianwei, Zeng Yinming. Myocardioprotective effects of the combination of ischemic preconditioning with hypothermia and crystalloid cardioplegia in immature rabbits. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).