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Integrins mediate the increase of concentration of intracellular free calcium in mouse eggs

Yueli Min, Zhang Lei, He Yaping, Zhang Jinhu, Xie Yimin, Zheng Yu, Zhang Li, Huang Ping, Wang Li, Liu Weixin

Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan Univeristy. Chengdu 610041, China；West China Hospital, Sichuan University. Chengdu 610041, China；Chengdu Institute of Family Planning. Chendu 610015, China

Abstract

For studying the effect of integrin on the [Ca~(2+)]_i of mouse eggs and its transmembrane signaling mechanism,zona-free mouse eggs were loaded with calcium probe Fluo-3/AM and the intensity of fluorescence of the eggs treated with different factors was measured through laser confocal microscopy. The results showed that the [Ca~(2+)], of zona-free mouse eggs was increased when the eggs were treated with RGD peptide, fibronectin (Fn) and anti-mouse integrin subunit α_6 and β_1 monoclonal antibodies, respectively. The [Ca~(2+)]_i, of the mouse eggs was also increased when the eggs were placed in calcium-free medium and treated with RGD peptide or Fn. The changes in the mouse egg [Ca~(2+)] _i caused by RGD and Fn were similar to those caused by sperm.

Key words: Integrins;Mouse oocyte;Intracellular calcium;Tyrosine kinase;Protein kinase C

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Citing This Article：

Yueli Min, Zhang Lei, He Yaping, Zhang Jinhu, Xie Yimin, Zheng Yu, Zhang Li, Huang Ping, Wang Li, Liu Weixin. Integrins mediate the increase of concentration of intracellular free calcium in mouse eggs. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).