Issue Archive

Levels of adrero medullin and proadrero medullin N-terminal 20 peptide in myocardium and aorta of spontaneously hypertersive ratsand Wstar- Kyoto rats

QI Yong Fen, Bu Dingfang, Shi Yanrong, Li Juxiang, Pang Yongzheng, Tang Chaoshu

Institute of Cardiovascular Disease，Peking University First Hospital，Beijing 100034；Department of Physiology，Peking University Health Science Center, Beijing 100083

Abstract

In this study，observed the levels of adrenomedullin(AD哟and proadrenomedullin Nterminal 20 peptide(PAMP) in myocardium and aorta of spontaneously hypertensive rats(SHRs) in comparison with Wistarkyoto(WKY) rats .Contents of ADM and PAMP were measured by radioimmunoassay(RIA) in plasma，myocardium and aorta .The amount of Pro' ADM mRNA of myocardium
and aorta was determined by competitive quantitative reverse transcription polymerase chain reaction(RT PCR).In SHRs the amounts of Pro, ADM mRNA of myocardium and aorta were 66.7%(P(0.01)and 73%(P(0.01)higher than those in WKY rat，respectively .In SHRs，the levels of ADM in plasma，myocardium and aorta were 29%，76.7%and 79%(all P(0.01)higher than those in WKY rats，ectively .The level of PAMP in SHRs was increased by 42.5%in plasma(P(0.01)，47.2%in myocardium(P(0.0.1)and 27.3%in aorta(P(0. 05) compared to WKY rats，tively .In addition，the ratio of ADM content to PAMP content in SHRs group was increased compared with that in WKY group(2.0士0.25ts1.64士0. 3 and 2. 2士0.18ts1.56士0. 28，in myocardium and aorta，respectively，P(0.01).These results suggest that ProADM gene expression is up- regulated and the increase in ADM and PAMP is different in SHRs .The significance of inconsistency of increase in ADM and PAMP in SHRs needs to be further investigated.

Key words: pathophysiology;Adrenomedullin;proadrenomedullin N terminal 20 peptide(PAMP);Spontaneously hypertensive rat;m RNA

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

QI Yong Fen, Bu Dingfang, Shi Yanrong, Li Juxiang, Pang Yongzheng, Tang Chaoshu. Levels of adrero medullin and proadrero medullin N-terminal 20 peptide in myocardium and aorta of spontaneously hypertersive ratsand Wstar- Kyoto rats . Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).