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Identification of differentially expressed genes of acinte hypoxia-treated Hep G2 cells and hypoxia-acclimatized Hep G2 cells

WANG Jin Hui , SHAN Ya- Jun, LONG Yu Wen, WU Lair Jun, YUAN Mao- Ling, ZHAO Zhen Hu, Wang Shengqi, Chen Jiapei

Institute of Radiation Medicane，Academy。 Military Medical Sciences，Beijing 100850

Abstract

To provide necessary information for further understanding of molecular mechanism of hypoxia accli matization，the differentially expressed genes of HepG2 cells exposed to normoxia，acute hypoxia7treated cells which were exposed to 1%oxygen for 48 h，and hypoxia7acclimatized HepG2 cells which were cultured for 6 circles of alternate low oxygen(1%oxygen for 24 h) and normal oxygen(21%oxygen for 24 h)，were identified respectively妙combining the suppression subtractive hybridization(SSH) and cDNA microarray .Thirty- seven
genes were expressed differentially in cells exposed to 1%oxygen for 48 h compared with those in cells exposed to normoxia .The expression of all these 37 genes was dower regulated，including the genes participating in cell
cycle，cell response to stimulus，and cell signal transduction，and cell cytoskeleton formation，the genes associated with transcription and cell metabolism，4 expressed sequence tags(ESTs)，and 12 genes of which the func-
tions are not known .There is a novel gene sequence，which has not been found in existing databases .There were only 6 genes differentially expressed in the hypoxia-acclimatized cells compared with cells exposed to normoxia，
including two mitochondrion genes，metalloprotease-1 gene，ferritin gene，thymosin beta-4 and TPTl genes.The expressions of mitochondrion ND4，ferritin，thymosin beta-4 and TPTl were up-regulated，while the expressions of mitochondrion NDl gene and metalloproease-1 gene were dower regulated .Cell tolerance to hypoxia increased after the cells were hypoxia7acclimatized .The different gene expression patterns of the acute hypoxia7treated cells
and the hypoxia7acclimatized cells may be related to the increased tolerance of the cells to hypoxia.

Key words: cell hypoxia;suppression subtractive hybridization;cDNA microarray;gene expression

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Citing This Article：

WANG Jin Hui , SHAN Ya- Jun, LONG Yu Wen, WU Lair Jun, YUAN Mao- Ling, ZHAO Zhen Hu, Wang Shengqi, Chen Jiapei. Identification of differentially expressed genes of acinte hypoxia-treated Hep G2 cells and hypoxia-acclimatized Hep G2 cells. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).