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Effects of embryonic neural stem cells and glial cell line derived neurotrophic factor in the repair of spinal cord i对ury

Sun Yong, Shi Jian, FU Sai- Li, LU Pei- Hua, XU Xiao- Mng

Departnrnt of Neurobiology , Shanghai Second Medical University, Shanghai 200025 ； Kentucky Spinal Cord In-jury Research Center，Department Neurological Surgery，University of Louisville，511 S. KY40292，USA

Abstract

The ability of implanted embryonic neural stem cells(NSCs) to improve survival，migration，and functional recovery following a compression spinal cord injury(SCI) was tested in adult rats.NSCs were isolated
from E14-16 rat cerebral cortex and SCI was produced妙using an aneurysm clip applicator applied to the 8th thoracic spinal cord according to method‘Dolan and Tator .Two weeks after the injury，NS Cs(4 u1‘1/10'
cells/ pJ) were injected into the lesion site.，The grafted NSCs were noted to survive and integrate with the host spinal cord 1 month after transplantation，which was demonstrated妙the presence of Hoechst 33342(a nuclear
dye) pre-labeled NSCs within and surrounding the lesion site .Some of these cells remained undifferentiated and were stained with nestin，a marker for NSCs .Transplanted NSCs migrated for at least 3 mm from the injury epi-
center towards both the rostral and caudal directions .Significant reduction in the lesion area(P<0. 05) and improvement in inclined plane(P<0. 05) and BBB locomotor rating scale(P<0. 05) were found in the cases that received implantation of NSCs，as compared with those that received vehicle .More importantly，when glial cell line-derived neurotrophic factor(GDNF;1 .5 pg/ pJ) was added to the transplants，further reduction in

Key words: spinal cord injury;;;

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Citing This Article：

Sun Yong, Shi Jian, FU Sai- Li, LU Pei- Hua, XU Xiao- Mng. Effects of embryonic neural stem cells and glial cell line derived neurotrophic factor in the repair of spinal cord i对ury. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).