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Reactive oxygen species and mitochondrial KATP serritive channels mediated cardioprotection induced扮TNI- a during hypoxia and reoxygenation

FU Chen, CAO Chun Mei, XIA qang, Yang Jun, LU Yuan

Department of Physiology，Zhejiang University School of Medicine，Hangzhou

Abstract

The aim of the present study was to testify whether the reactive oxygen species and mitochondrial ATP- sensitive potassium(KATP) channels were involved in the cardioprotection induced妙tumor necrosis factor a (TNr- a) in the cultured neonatal ventricular myocytes suffered from 12 h of hypoxia and 6 h of reoxygenation.We tested the release of lactate dihydroge nase(LDH) and manganese superoxide dismutase(Ma-SOD) with spectrophotometay·It was shown that pretreatment with TNF=a(10，50，100，or 500 U/ ml) significantly in-
creased the Mar SOD activity and reduced LDH release in the neonatal ventricular myocytes subjected to hypoxia and reoxygenation·Pretreatment with NAC(1 mmol/ L)，antimycin A(50 p mol/ L)，2- MPG(400 pmol/L)，pectively，attenuated the increase in MLrSOD activity and reduction of LDH level induced by TNF=a in ventricular myocytes .Diazoxide(50 umol/L，a selective opener of
the mitochondrial KATP channel，decreased the LDH release of the myocytes su ected to hypoxia and reoxygenation，which could be abolished by pretreatment with NAC(1 mmol/ L) or 5- HD(100 p mol/ L).These results suggest that oxygen radical signals and mitochondrial KATP channels are involved in the cardioprotection induced by TNF#alpha#

Key words: myocytes;tumor necrosis factor a(TNr- a);hypoxia/ reoxygenation; reactive oxygen ;;

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Citing This Article：

FU Chen, CAO Chun Mei, XIA qang, Yang Jun, LU Yuan. Reactive oxygen species and mitochondrial KATP serritive channels mediated cardioprotection induced扮TNI- a during hypoxia and reoxygenation. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).