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Effects of #alpha# methyl (4- tetrazolyl phenyl)glycine on the induction of hippocampal ischemic tolerance in the rat

FENG Rong- Fang, LI Wen-Bin, LIU Hui- Qing, LI qing- Jun, CHEN Xiao- Ling, ZHOU Ai- Min，Zhao Honggang, Ai jie, Ai Jie

Department of Pathophysiology，Institute of Basic Medicine，Hebei Midical Universiity，Shijiazhuang 050017

Abstract

To explore the role of metabotropic glutamate receptor 2/ 3(mG1uR 2/ 3) in the induction of brain ischemic tolerance(BIT)，the influences of mG1uR2/ 3 antagonist a methyl-(4- tetrazolyl- phenyl)glycine (MTPG) on the induction of BIT and expression of glial fibrillaiy acidic protein(GFAP) in the hippocampus were observed using thionin staining and GFAP immunohistochemical staining in a rat brain ischemic model with four vessel occlusion(4 VO) .Fifty-four rats，of which bilateral vertebral arteries were occluded permanently by
electrocauteiy，were divided into 5 groups:(1)sham operated group(n一8):the bilateral carotid common arteries(BCCA) were separated，but the blood flow was not blocked;(2) ische mia group(n一8):the blood flow of BCCA was blocked for 8 min;(3) ischemic preconditioning(IP) group(n一8):the blood flow of BCCA was
occluded for 3 min as a cerebral ischemic preconditioning(CIP)，and then the rats were exposed to an 8- min brain ischemic insult 24 h after the CIP;(4) MTPG+IP group(n一22):MTPG was administered 20 min before the CIP，then the rats were exposed to an 8- min brain ischemia insult 24 h after the CIP .In order to examine dosage dependency in the effect of MTPG，4 dosages of MTPG(0.4，0.2，0. 04 and 0. 008 mg) were administered.

Key words: cerebral ischemic preconditioning;metabotropic glutamate receptors;a methyl-(4- tetrazolyl- phenyl) glycine;hippoca mpus ;

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Citing This Article：

FENG Rong- Fang, LI Wen-Bin, LIU Hui- Qing, LI qing- Jun, CHEN Xiao- Ling, ZHOU Ai- Min，Zhao Honggang, Ai jie, Ai Jie. Effects of #alpha# methyl (4- tetrazolyl phenyl)glycine on the induction of hippocampal ischemic tolerance in the rat. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).