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Expression of #beta#_(2) adrenergic receptor and its effect on the proliferation of neonatal rat cardiac fibroblasts

Yin Feng, LU Zhi- Zhen, Han Qide, ZHANG You Yi

Institute of Vascular Medicine，Peking Unizrrsity Third Hospital，The Reference Laboratory of Ministry of Education for Moleculer Cardiovasology，Beijing 100083-

Abstract

The expression of f}-adrenergic receptor subtypes and its effect on neonatal rat cardiac fibroblast proliferation were investigated妙radioligand binding assay and[3 H rthymidine incorporation analysis，respec-
tively .The results indicated that there was no significant difference in the f}-adrenergic receptor density(Bmax)and affinity(KD) between cardiomyocytes and cardiac fibroblasts.The[~(125)I rpindolol competitive inhibition
curves(ICI 118551 and CGP 20712A) were significantly better fit in a one-site model in membrane preparation of cardiac fibroblasts .in cultured cardiac fibroblasts，0. 1 p mol/ L isoproterenol-induced[~(3)Hthymidine incor
poration was completely inhibited by a selective O2 AR antagonist ICI 118551，or a non- selective f}- AR antagonist propranol of，but not妙CGP 20712A，a selective p,一AR antagonist .These results suggest that isoproterenol-in-
duced cardiac fibroblast proliferation is mediated妙p2 AR，the preponderant f- AR subtype in cardiac fibroblasts.

Key words: adrenergic receptor;radioligand binding assay;Cardiac fibroblasts;proliferation

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Citing This Article：

Yin Feng, LU Zhi- Zhen, Han Qide, ZHANG You Yi. Expression of #beta#_(2) adrenergic receptor and its effect on the proliferation of neonatal rat cardiac fibroblasts. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).