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Gender--dependent difference of NF--#kappa#B expression in the hippocampus of prenatally stressed offspring rats

Li Hui, Zhu Zhongliang, Jia Ning, Bai Zhuanli, Cai Qing, Chen Rui, Song Tianbao, Liu Jiankang

Department of Human Anatomy and Histology-Embryology,School of Medicine, Xi'an Jiaotong University.Xi'an 710061,Shaanxi；China；Department of Physiology and Pathophysiology, School of Medicine, Xi'an Jiaotong University.Xi'an 710061,Shaanxi

Abstract

In this study, immunohistochemistry and Western blot were used to determine whether the expression of NF-#kappa#B in the hippoeampus of prenatally stressed offspring rats is gender-dependent. The results were as follows： In the female offspring rats, the expressions of D65 in the hippoeampal dentate gyms in mid-term stress （MS） and late-term stress （LS） groups were significantly less than that in the control group （P〈0.01）. There was a significant difference between MS and LS groups （P〈0.01）. The expressions of p50 in all regions of hippoeampus in MS and LS groups were significantly more than that in the control group （P〈0.01）. A significant difference was also present between MS and LS groups （P〈0.01）. In the male offspring rats, the expressions of D65 in the hippoeampal dentate gyrus in MS and LS groups were evidently more than that in the control group （P〈0.01）. There was a significant difference between MS and LS groups （P〈0.01）. The expressions of p50 in all regions of hippocampus in MS and LS groups were significantly less than that in the control group （P〈0.05, P〈0.01）. There was also a significant difference in p65 expression between MS and LS groups （P〈0.01）. In addition, in the control group the expressions of p65 in the hippoeampal dentate gyms of female offspring rats were significantly more than that of male ones （P〈0.01）. However, in LS group the expressions of p65 in the hippoeampal dentate gyms of female offspring rats were significantly less than that of male ones （P〈0.01）. Moreover, there was no significant difference in p65 expression between female and male offspring rats in MS group. In the control group the gender difference in the expression of p50 was only observed in hippocampal CA1 （P〈0.01）. The expressions of p50 in all regions of hippocampus of female offspring rats were significantly more than that of male ones in LS group （P〈0.01）. There was no significant difference in p50 expression between female and male offspring rats in MS group. The results of Western blot were similar to those of immunohistochemical study. These results indicate that prenatal stress in different gestational periods significantly affects the expressions of p65 and p50 in hippocampus, and this effect is gender-dependent. This may be one of the mechanisms underlying the gender difference in the ability of learning and memory of the prenatally stressed offspring rats.

Key words: NF-#kappa#B;prenatal stress;sexual distinction;Hippocampus

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Citing This Article：

Li Hui, Zhu Zhongliang, Jia Ning, Bai Zhuanli, Cai Qing, Chen Rui, Song Tianbao, Liu Jiankang. Gender--dependent difference of NF--#kappa#B expression in the hippocampus of prenatally stressed offspring rats. Acta Physiol Sin 2006; 58 (6): (in Chinese with English abstract).