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Investigation on spontaneous electrical activity of murine embryonic heart using microelectrode arrays

Duan Yaqi, Tang Ming, Liang Huamin, JÜrgen Hescheler

Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Pulmonary Disease Laboratory of Ministry of Health of China. Wuhan 430030, China；Institute of Neurophysiology, University of Cologne, Cologne D-50931, Germany

Abstract

In our studies, we have applied a novel tool----microelectrode arrays (MEA), to investigate the electrophysiological properties of murine embryonic hearts in vitro. The electrical signals were recorded from the areas of the heart adhering to the 60 MEA electrodes, being called field potentials (FPs). As an extracelluar recording, the waveform of the FP appeared similar to a reversed action potential obtained from single cell by whole cell current clamp and the FP duration was comparable with the action potential duration. To study propagation of spontaneous electrical activity, we have compared the occurrence time of FPs recorded from different electrodes. It is shown that there was already an apparent A-V delay [(50.21±9.7) ms] at day 9.5 post coitum (E9.5) when heart was still tubular-like and atrium and ventricle were not separated anatomically, while occurence of FP at different electrodes of ventricular area were almost synchronous. Further, we looked into the modulation of spontaneous electrical activity during cardiac development: at E9.5 of embryonic development, 1 #mu#mol/L of isoproterenol (Iso) increased beating frequency by (34.04±7.31)%, shortened the A-V delay by (20.00±6.44)% and prolonged FP duration. In contrast, 1 #mu#mol/L of carbachol (CCh) slowed down beating frequency by (42.32±5.36)%, A-V conduction by (26.00±4.81)% and shortened FP duration; however at late stage (E16.5), the regulatory effect of Iso and CCh was strengthened. Therefore we conclude that cardiac conduction system is already established at E9.5 when the four-chambered heart is not formed yet and the regulation of spontaneous activity by sympathetic and para-sympathetic system is gradually matured during cardiac development.

Key words: Microelectrode arrays;Field potential;Action potential

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Citing This Article：

Duan Yaqi, Tang Ming, Liang Huamin, JÜrgen Hescheler. Investigation on spontaneous electrical activity of murine embryonic heart using microelectrode arrays. Acta Physiol Sin 2006; 58 (1): (in Chinese with English abstract).