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Effects of D_(1) and D_(2) dopamine receptor agonists and antagonists on cerebral ischemia/reperfusion injury

Zong Xuemei, Zeng Yinming, Xu Tie, Lo Jiannong

Center of Emergency, Affiliated Hospital of Xuzhou Medical College. Xuzhou 221002, China；Jiangsu Province Key Laboratory of Anesthesiology. Xuzhou 221002, China

Abstract

Gerbil forebrain ischemia/reperfusion(I/R) injury model was used to study the effects of D_1and D_2 receptor agonists and antagonists on neuronal apoptosis of hippocampal CA1 area. All animals weretested for habituation deficits in an open field test on the 1st, 3rd and 7th days after reperfusion. The animalswere then killed, and brains underwent paraffin embedding for hematoxylin-eosin staining, in situ terminaldeoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) staining and immunohisto-chemistry (bax, bcl-2). The result of open field test showed that the I/R group was significantly impaired(higher activity scores) when compared with the control group. Pretreatment with pergolide significantly re-duced this habituation impairment. Forebrain ischemia for 5 min resulted in extensive CA1 apoptosis on the3rd and 7th days after I/R injury.

Key words: Cerebral ischemia/reperfusion injury;Apoptosis;Dopamine;Gerbil;Bcl-2;Bax

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Citing This Article：

Zong Xuemei, Zeng Yinming, Xu Tie, Lo Jiannong. Effects of D_(1) and D_(2) dopamine receptor agonists and antagonists on cerebral ischemia/reperfusion injury. Acta Physiol Sin 2003; 55 (5): (in Chinese with English abstract).