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Role of摄滋 actin nncrolilamert in hypos nntic membrane stretch induced increase in mucarirac current of少。内gastric my

WANG Zuo Yu, YU Yongr Chun, CUI Yi- Feng, LI Lin, GUO Hui-Shu, LI Zai Liu, Xu Wen Xie，

Research Laboratory of Digestive Physiology and Research Center of Affiliated Hospital，Yanbian University College of Medicine，Yanji 133000

Abstract

To investigate the relationship between cytoskeleton and hypos motic membrane stretch-induced increase in muscarinic current，the role of actin microfilament in hyposmotic membrane stretc卜induced increase in muscarinic current was studied with the whole-cell patch clamp technique in guinea-pig gastric myocytes .In this study，the muscarinic current was induced by carbachol(50 umol/ L) or GTPYB(0.5 mmol/L).The results showed that hyposmotic superfusate(202mOsmol/ L) increased carbachol-induced current(lCCs) by 145士27%and increased GTPYS-induced current by 183士30%;but in the presence of cytochalasirr B(cyt- B，20 umol/ L)，an actin cytoskeleton disrupter，posmotic membrane stretch increased 1CC6 by 70士6%.However，hypos motic membrane tretch induced increase in 1CC6 was potentiated to 545士81% by phalloidin(20 umol/ L)，an actin microfilament stabilizer.The results demonstrated that hyposmotic membrane stretch increased the muscarinic currents induced by carbachol or GTPYS and that the actin microfilament is involved in the process n guinea7pig gastric myocytes.

Key words: muscarinic currents;gastric myocytes;stretch;actin microfil ament

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Citing This Article：

WANG Zuo Yu, YU Yongr Chun, CUI Yi- Feng, LI Lin, GUO Hui-Shu, LI Zai Liu, Xu Wen Xie，. Role of摄滋 actin nncrolilamert in hypos nntic membrane stretch induced increase in mucarirac current of少。内gastric my. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).