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Recombinant human interleukirr 10 inhibits proliferation of vascular smooth muscle cells sti mutated场advanced glycation end products and neoirnti ma hyperplasia after carotid in3 ury in the rat

OU YANG Ping, PE NG Li-Sheng, Yang Hong, PENG Weir Lie, WU Wen Yan, XU Air Long

Department of Biochemistry，School of Life Science，Sun Yat-sen University，Guangzhou 510275，China

Abstract

The purposes of this study was to determine the effects of recombinant human interleukirrl0(rhIL 10) on proliferation of vascular smooth muscle cells(VS MCs) stimulated by advanced glycation end products(AGE) and neointima by-
perplasia after rat carotid arterial injury .Rat aortic VS MCs were cultured and treated with rhIL-10 or AGE respectively，and then co-treated with rhIL 10 and AGE.Proliferation of VSMCs was quantified by colormetric assay.Cell cycle analysis was performed by flow cytomertry .Sprague-Dawley rats were treated with recombinant human IL-10(rhIL 10) for 3 d after carotid arteries injury .The ratio of neointima to media area at the site of arterial injury was measured 28 d after balloon injury .The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti7p44/42 phospho- MAPK antibody .Compared to control，AGE stimulated VSMCs proliferation.rhIL-10 alone had no effect on VSMCs growth.With AGE stimulation，rhIL 10，at dose as low as 10 ng/ ml，inhibited VS MCs growth(P(0. 05).The cell number in Go/ G,
phase of AGE and rhIL 10 co-treatment group was higher than that of AGE treatment alone(P(0. 01)by flow cytometry analysis .Compared with the control group of neointima hyperplasia in rats，the ratio of neointima to media area of recombinant human IL-10 group was reduced by 45%(P(0. 01).The p44/42 MAPK activity was significantly enhanced by AGE .The AGE effects were opposed by rhIL- 10.The anti7inflammatory cytokine rhIL-10 inhibits AGE induced VSMCs
proliferation .Recombinant human IL 10 also inhibited neointima hyperplasia after carotid artery injury in rats.The results suggest the possibility that recombinant human IL 10，as a potential therapeutic approach，prevents neointimal hyperplasia.

Key words: pathology;interleukin-10;muscle;smooth;vascular;;

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Citing This Article：

OU YANG Ping, PE NG Li-Sheng, Yang Hong, PENG Weir Lie, WU Wen Yan, XU Air Long. Recombinant human interleukirr 10 inhibits proliferation of vascular smooth muscle cells sti mutated场advanced glycation end products and neoirnti ma hyperplasia after carotid in3 ury in the rat. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).